凱普生物(300639.SZ):公司及子公司獲得一項發明專利授權
格隆匯9月30日丨凱普生物(300639.SZ)公佈,公司以及子公司在2024年9月獲得一項發明專利授權,發明專利名稱為一種磁珠法提取幹血斑基因組DNA的試劑盒及其應用。
本發明涉及核酸提取的技術領域,提供了一種磁珠法提取幹血斑基因組DNA的試劑盒及其應用。該試劑盒由核酸釋放劑、裂解液、磁珠、洗滌液Ⅰ、洗滌液Ⅱ和洗脱液組成。本發明在核酸釋放劑中加入NaOH和鹽酸胍,能夠快速裂解細胞,釋放出核酸,同時在核酸釋放劑中加入DTT對核酸純度有明顯的提升。此外,在裂解液中加入月桂基二甲基氧化胺也可以提高核酸的濃度和純度。上述試劑盒可以高效提取幹血斑樣本中的核酸,具有純度高、收率高、操作簡單、用時短、全自動、高通量和成本低等優點,可滿足大批量樣本的提取要求。
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